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RNA cyclisation: circular RNA is produced by joining the two ends within the RNA molecule by T4 RNA ligase 1 (T4 Rnl1) or T4 RNA ligase 2 (T4 Rnl2) catalysing the joining of 5'-phosphate and 3'-hydroxyl groups at the RNA terminus
EGFP mRNA is a messenger RNA molecule capable of expressing enhanced green fluorescent protein (EGFP)
Excellent coupling efficiency and yield
} DMT-2’-F-dG(iBu)-CE-Phosphoramidite is a 2'-fluoro (2'-F) phosphoramidite monomer used for solid-phase oligonucleotide synthesis
a 5’-dimethoxytrityl (DMT) group for 5'-hydroxyl protection
RNase Inhibitor NMP RNA cyclisation: circular RNA isProduct Information Ribonuclease Inhibitor, Human Placenta is a recombinant human placental protein with specifically inhibits broad spectrum RNase such as RNase A, RNase B and RNase C. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H, Taq DNA polymerase, M MLV Reverse Transcriptase and T7 RNA Polymerase. The 50kDa protein exerts its inhibitory effect by noncovalently binding to RNases at 1: 1 ratio. The Ki value for binding of
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